再来菜菜的问问

taomasxu

大家好．．．就是关于头像的问题．．．下载后到底压缩到那里？解压缩后的文件要不要改变格式？还是就是ＤＤＴ文件？
大家帮手呀．．．．大家都要顶呀．．．其实还是有太多的人不会．．也好教教他们．．．谢谢了～～～～
还有下载哪个比较好？大家都说下．．．特别是已经下载了的～～～ 0=042 0=042 0=042 0=042 0=042 0=042 0=042

滚滚

要照片才告诉你。。

taomasxu

The kinetic behavior of mushroom tyrosinase　was studied during the oxidation of l-tyrosine and　l-DOPA. Under the conditions employed in the　present study, the progress curves for the enzyme at　different concentrations of l-tyrosine and l-DOPA
have been determined. When the diphenolase activity　of mushroom tyrosinase was assayed by using　l-DOPA as substrate, the reaction immediatelyreached a steady-state rate (Fig. 1, curve 1). It was　a straight line which passed through the original
point of the coordinate, and the slope of the straight　line was the initial rate (v0). The relationship of　the initial rate and the concentration of substrate　(l-DOPA) followed Michaelis–Menten kinetics. Kinetic　parameters for the diphenolase activity of
mushroom tyrosinase are shown in Fig. 4 (line 0) as　Lineweaver–Burk plot. The apparent Michaelis constant　(KDm) is 0.68±0.02mM and the apparent maximum　velocity (VDm) is 151.9±2.5
M/min. When the　monophenolase activity of mushroom tyrosinase was
assayed by using l-tyrosine as substrate, a remarkable　lag period, characteristic of monophenolase activity,　was observed simultaneously with the appearance of　the first stable product, dopachrome (Fig. 1, curve4). The system reached a constant rate after the
lag period, which was estimated by extrapolationof the linear portion of the product accumulation　1662 L.-P. Xie et al. / The International Journal of Biochemistry & Cell Biology 35 (2003) 1658–1666　curve to the abscissa (Esp´ın & Wichers, 1999). After
the reaction system reached the steady state, the　curve of product increased straightly with increasing　the reaction time. And the slope of the line denoted　the steady-state rate. The relationship of the　steady-state rate with the concentration of substrate
(l-tyrosine) followed Michaelis–Menten kinetics too.　Kinetic parameters for the monophenolase activity　of mushroom tyrosinase are exhibited in Fig. 5 (line0) as Lineweaver–Burk plot. The apparent Michaelis　constant (KMm) is 0.29 ± 0.03mM and the apparent
maximum velocity (VMm ) is 36.5 ± 1.5
M/min.

taomasxu

要什么照片呀？？／

taomasxu

是不是这个呀？是本人的呀

taomasxu

顶～～～～～大家说话呀～～～，哎．．．原来还在睡觉呀．．不过睡醒了记得告诉我呀 0=042

taomasxu

顶～～～～～使劲顶～～～不信了饿

滚滚

置顶帖里都有吧。。